0 to 4. Exhibit with us {{>currencyLabel}} Back. This assay measures the two major sugars in wine, glucose and fructose. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. D2. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Data were expressed as the mean percent viable cell vs. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. Thin layer chromatography (TLC) was. Percent Transmission Absorbance Competitor 0. Molecular Devices Spectramax 340PC instructions. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 190–850 nm <± 1. Statistical analysis. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. Buffer (100 mmol L –1acetate buffer, pH 5. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. SpectraMax Multi-Mode Microplate Readers. If depot service is the best option for you we will assist you with logistics to help you keep. 340/70 nm. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. The initial rate of hydrolysis was determined using the early linear region of. or microplate reader (e. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Microplate Readers. It includes both a lamp and a new lamp holder. Upgradeable modules are also available including western blot, cell imaging, and fast. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Crowder, Ziqian Zeng, Alissa N. 2. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Donor: 620 nm. One plate carrier adapter is provided with the instrument. Not. Samples were plated in duplicate (r=0. Plates were washed with 1× KP solution and blocked with. 312 gain calibration failed. Molecular Devices SpectraMax M5e Microplate Reader $26,500. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Values were determined according to a standard curve (log/log curve-fit). VersaMax reads 96-well and strip-well plates. (Shiga, Japan) and kept for 30 min in the dark. Acceptor: 665/10 nm. The inhibition percentage was determined by linear regression through ranges of 20 to 30. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. One plate carrier adapter is provided with the instrument. Versteegh, Yoran H. Microplate Spectrophotometer Operator’s Manual. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. using SpectraMax i3 (Molecular devices, San Jose, USA). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 4) was followed according to Vanacker et al. Samples with OD values of less than 0. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. , Fremont, CA, USA). Products : SpectraMax Plus 384 reader,. , Sunnyvale, CA). Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices Corporation 1311. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. 5 mL sample or standard solution was well mixed with 0. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. 85%. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. (7. . 304 signal level saturation. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. 6 (h) x 22. Pharmacokinetic and pharmacodynamic analysis. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 0200-6117. 0 1. 2 g/L solution; dilute 1:10 in HBSS. Fibrin clots were formed as described above with some modifications 46. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. The absorbances were transformed to velocity of substrate consumption (nmol/min). Wells were washed three times. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. #N207) LAL Reagent Water (Lonza cat. Cross sections of mouse skin was prepared and stained as previously described . Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices SpectraMax Gemini XS Microplate Reader. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Molecular Devices. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). A. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Bioz Stars score: 86/100, based on 1 PubMed citations. , Sunnyvale, CA). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The contents of the. SpectraMAX 340 microtiter plate reader. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. SPECTRAMAX 340. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 2 were considered negative for IgM antibody. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. Description. 5. 5. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. The plates were incubated for 4 additional hours at 37°C. Each experiment was performed in duplicate and repeated at. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Cell proliferation was measured using the MTT method. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Details. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Immunofluorescence and Aggregation Analysis. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Analysis of ALA by Ehrlich’s Reagent Assay. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Immunofluorescence and Aggregation Analysis. Introduction. 1% PEG 8000 at 37°C. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. The concentration of TNFα in the experimental. The sensitivities of the hormones are Prolactin: 0. 0. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. The light features a slim profile to prevent. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Read one sample or up to 384. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Unit powers. 5. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. , Sunnyvale, CA). • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. 316 grating motor driver faulty. SpectraMax Multi-Mode Microplate Readers. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Details. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. G. Clotting times (mean ± SD) for rSimukunin and rSV. A 0. Fully tested with Warranty. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. reader (Molecular Devi ces). Nitric Oxide Assay. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. One plate carrier adapter is provided with the instrument. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). 10× trypsin 0. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Six wells were assayed for each set of conditions and stadard deviations. Description 1. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Stat Fax 4700 Microstrip Reader. Molecular Devices SpectraMAX 340 Microplate Reader. 5 ng/ml; estradiol: 10 pg/ml. 2. StakMax Microplate Handling Stacker. Setup recommendations for SpectraMax. Fig. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). ). SpectraMAX 340 plate reader (Molecular Devices). SpectraMax 340 from Molecular Devices). Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Figure 1. The SPECTRAmax 340PC can measure. , Sunnyvale, CA). TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. well, and strip-well microplates. 0. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Software (SoftMax Pro ® software or comparable plate reader software). Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Page: 1/6. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置,请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整,以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. #ABS PLUS) Methods. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 003 OD Stray light ≤ 0. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). control. 3 mm. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . The first time you replace an OEM lamp you must use the N-34-MLA. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Immunofluorescence and Aggregation Analysis. 303 unable to cal dark current. Binding of capture antibodies to polystyrene beads and biotinylation. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 305 reference level saturation. 5 mL of 1 M acetate buffer (pH 4. 3. mums. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Tunable for filterless flexibility. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. View the Molecular Devices website. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Donor filter: 616/10 nm. Novak, Nathan J. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. AoSMCs were seeded into 6-well culture plates. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Manager: Yinghua Chen, PhD Contact Email: yinghua. 0. FACS analysis. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 315 can't find zero order. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Detection limits. Each experiment was performed in duplicate and repeated at. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. used. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Manager: Yinghua Chen, PhD Contact Email: yinghua. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 7. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 06 ng/mL; FSH: 0. Binding affinity for denosumab. 303 unable to cal dark current. SpectraMax Multi-Mode Microplate Readers. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). reader (Molecular Devi ces). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. HTRF-compatible SpectraMax Multi-Mode Readers. 2. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. TDP-43 stress granular cells were treated with 70% ice-cold. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 5. Donor: 616/10 nm. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. DNA extraction from blood meal ELISA homogenate. Anti-proteolytic activity. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. 313 reference gain check fail. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. 5. Please see photos. The plate was covered and incubated for one hour at room temperature. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Labsystems Luminoskan RT Microplate Reader. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. SpectraMAX 340. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. This test was performed in duplicates. (1993). Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 0200-7027. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. edu Contact Phone: 216-368-8962. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Gerritsma,B. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. These robust readers have been placed in labs from Antarctica to the. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). A. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. 3 0. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. application or budget. 7 macrophages were obtained from ATCC (Manassas, VA). Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Customer Transformation Hub. TDP-43 stress granular cells were treated with 70% ice-cold. Call 1-800-745-2710. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Quick View. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 3: The cuvette chamber. 312 gain calibration failed. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Other technologies such as imaging. 9000-0756. 2 - 3. Molecular Devices SpectraMax M5e Microplate Reader. . Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. 001/min at 425 nm. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 04. MCE has not independently confirmed the accuracy of these methods. Temperature Range: Ambient + 4 °C up to 45 °C. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. This reader only allows high performance HTRF measurement when assays are run in. Microplate Readers. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to.